Studying GST-tagged proteins can be as simple as that!

Are you experimenting with GST-tagged proteins? To experience how GST-tagged proteins simplify protein-protein interaction analysis!

Why study GST tagged proteins?

Glutathione thiotransferase (GST) is a 26KDa protein of the size naturally present in eukaryotic cells. GSTs can catalyze the binding of exogenous substances to glutathione (GSH), thus detoxifying many environmental toxins including chemotherapy drugs, residual drugs, herbicides and carcinogens. Many researchers have integrated the GST DNA sequence into an expression vector to produce a GST-tagged fusion protein. GST is fast-stabilized and has good solubility, thus promoting the expression and dissolution of the fusion protein. In addition, protein binding and detection can also be performed using the combination of GST and its substrate GSH. Currently, GST-tagged proteins are used in a variety of research techniques, such as pull-down, affinity chromatography, and ELISA. For the convenience of researchers, Nicoya Lifesciences has also introduced a GST function chip for immobilizing GST-tagged proteins, making your surface plasmon resonance analysis faster and easier.

What is SPR technology?

Surface Plasmon Resonance (SPR) can be used to analyze many types of intermolecular interactions, including proteins and proteins, proteins and small molecules, proteins and nucleic acids, proteins and aptamers, proteins and lipids, proteins and sugars. , sugars and sugars. Traditional experimental techniques such as CoIP, ELISA, and fluorescence resonance energy transfer often require cumbersome labeling steps or complex and expensive instruments. Most techniques only capture the binding or affinity between molecules. In recent years, more and more researchers have begun to choose SPR technology to detect real-time biomolecules of interest. SPR is a label-free analytical technique for the quantitative analysis of interactions between two biomolecules. Compared with other technologies, SPR can not only determine the affinity, but also kinetic analysis of the interaction between two biomolecules to obtain the binding rate and dissociation rate.

Chip selection in SPR experimental design

When starting an SPR experiment, you first need to choose the SPR chip that is right for you. The chip consists of a glass medium, nano gold particles and functional modifications of the particle surface. The choice of chip depends on the ligand you are using, the property of the molecule that needs to be attached to the surface of the chip. The GST chip can greatly simplify the immobilization process of GST-tagged proteins, and glutathione (GSH) on the surface of the chip can directly capture GST-labeled ligands for fixation.

Dynamic binding analysis using OpenSPR GST chip

The figure below shows the immobilization and kinetic binding analysis of GST-tagged proteins using the OpenSPR GST chip. GST-ubiquitin was immobilized on a GST chip and the binding between the Ubiqutin antibody and Ubiqutin was analyzed using the OpenSPR instrument with a KD value of 95.3 nM.

GST-ubiquitin ligand is immobilized to GST chip

Ubiqutin antibody concentration was 7.4nM, 22.3nM, 66.7nM

The role of SPR technology in life science research is becoming more and more important, and has attracted much attention in many medical and medical research. Articles with SPR data are also among the best journals. I believe that SPR data will make your Paper more persuasive and influential!

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