(1) When bacteria are separated, the concentration of 50 U/ml nystatin is added to the medium to inhibit the growth of mold and yeast.
(2) When separating actinomycetes, adding 0.05% sodium dodecyl sulfate (SDS) to the sample not only inhibits the growth of bacteria, but also activates the germination of actinomycetes. Adding norfloxacin (5 mg/L) + nystatin (50 mg/L) + penicillin (0.8 mg/L) can also effectively inhibit bacteria and fungi without affecting the growth of actinomycetes.
(3) When mold and yeast are isolated, 30 U/ml of penicillin, streptomycin and tetracycline are added to the medium to inhibit the growth of bacteria and actinomycetes.
(4) When Rhizopus and Mucor are isolated, since the hyphae of these microorganisms easily spread into pieces, it is difficult to obtain purified colonies, and usually 0.1% sodium deoxycholate or sorbitol is added to the culture medium to prevent the spread of hyphae. The colonies grow small and tight.
(2) When separating actinomycetes, adding 0.05% sodium dodecyl sulfate (SDS) to the sample not only inhibits the growth of bacteria, but also activates the germination of actinomycetes. Adding norfloxacin (5 mg/L) + nystatin (50 mg/L) + penicillin (0.8 mg/L) can also effectively inhibit bacteria and fungi without affecting the growth of actinomycetes.
(3) When mold and yeast are isolated, 30 U/ml of penicillin, streptomycin and tetracycline are added to the medium to inhibit the growth of bacteria and actinomycetes.
(4) When Rhizopus and Mucor are isolated, since the hyphae of these microorganisms easily spread into pieces, it is difficult to obtain purified colonies, and usually 0.1% sodium deoxycholate or sorbitol is added to the culture medium to prevent the spread of hyphae. The colonies grow small and tight.
The medium generally used for the separation of single-purpose microorganisms contains inhibitors for inhibiting other microorganisms. These specialized inhibitors are cumbersome to prepare in a small laboratory, and are now available as a finished product, as long as the basal medium is directly added. Such as ampicillin, mainly used to isolate Aeromonas hydrophila.
1, bacterial culture medium
Formula 1 Beef Cream Agar Medium Beef Cream 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g,
Water 1000 ml
Add 100 ml of water to the beaker, add beef paste, peptone and sodium chloride, mark with a crayon on the outside of the beaker, and heat on a fire. After the components in the beaker are dissolved, add agar and stir constantly to avoid sticking to the bottom. After the agar is completely dissolved, the water is replenished, and the pH is adjusted to 7.2-7.6 with 10% hydrochloric acid or 10% sodium hydroxide, and the mixture is placed in each test tube, and the cotton plug is added and sterilized by autoclaving for 30 minutes.
Formula 2 Potato Medium
Take 250 grams of fresh beef heart (removing fat and blood vessels), finely knead it into minced meat with a knife, add 500 ml of distilled water and 5 g of peptone. Make a mark on the beaker, boil, and simmer for 2 hours. After filtration, the minced meat was dried and the pH of the filtrate was adjusted to about 7.5. Add 10 ml of broth and a small amount of dried dried beef heart to each tube, sterilize and set aside.
Formula four rhizobium culture medium
Glucose 10 g Dipotassium hydrogen phosphate 0.5 g Calcium carbonate 3 g Magnesium sulfate 0.2 g Yeast powder 0.4 g Agar 20 g Water 1000 ml 1% crystal violet solution 1 ml
First, add the agar to the water and boil it, then add the other components separately, stir to dissolve, then dispense, sterilize, and set aside.
1, bacterial culture medium
Formula 1 Beef Cream Agar Medium Beef Cream 0.3g, peptone 1.0g, sodium chloride 0.5g, agar 1.5g,
Water 1000 ml
Add 100 ml of water to the beaker, add beef paste, peptone and sodium chloride, mark with a crayon on the outside of the beaker, and heat on a fire. After the components in the beaker are dissolved, add agar and stir constantly to avoid sticking to the bottom. After the agar is completely dissolved, the water is replenished, and the pH is adjusted to 7.2-7.6 with 10% hydrochloric acid or 10% sodium hydroxide, and the mixture is placed in each test tube, and the cotton plug is added and sterilized by autoclaving for 30 minutes.
Formula 2 Potato Medium
Take 250 grams of fresh beef heart (removing fat and blood vessels), finely knead it into minced meat with a knife, add 500 ml of distilled water and 5 g of peptone. Make a mark on the beaker, boil, and simmer for 2 hours. After filtration, the minced meat was dried and the pH of the filtrate was adjusted to about 7.5. Add 10 ml of broth and a small amount of dried dried beef heart to each tube, sterilize and set aside.
Formula four rhizobium culture medium
Glucose 10 g Dipotassium hydrogen phosphate 0.5 g Calcium carbonate 3 g Magnesium sulfate 0.2 g Yeast powder 0.4 g Agar 20 g Water 1000 ml 1% crystal violet solution 1 ml
First, add the agar to the water and boil it, then add the other components separately, stir to dissolve, then dispense, sterilize, and set aside.
2. Actinomycetes medium
Formulation 1 Starch Agar Medium (Gao's Medium)
Soluble starch 2 g potassium nitrate 0.1 g dipotassium hydrogen phosphate 0.05 g sodium chloride 0.05 g magnesium sulfate 0.05 g ferrous sulfate 0.001 g agar 2 g water 1000 ml first put the starch in a beaker, and mix it with 5 ml of water into a paste After that, pour 95 ml of water, stir well, and add other medicines to dissolve it. Make a mark outside the beaker, add agar when heated to boil, stir constantly, wait until the agar is completely dissolved, make up the water loss. Adjust the pH to 7.2 ~ 7.4, sterilize after dispensing, and set aside.
Formula 2 Flour agar medium
Flour 60 g Agar 20 g Water 1000 ml Flour the water into a paste, add water to 500 ml, and cook on a simmer for 30 minutes. Another 500 ml of water, add agar, heat and boil until dissolved, mix the two liquids, add water, adjust the pH to 7.4, dispense, sterilize, spare.
Formulation 1 Starch Agar Medium (Gao's Medium)
Soluble starch 2 g potassium nitrate 0.1 g dipotassium hydrogen phosphate 0.05 g sodium chloride 0.05 g magnesium sulfate 0.05 g ferrous sulfate 0.001 g agar 2 g water 1000 ml first put the starch in a beaker, and mix it with 5 ml of water into a paste After that, pour 95 ml of water, stir well, and add other medicines to dissolve it. Make a mark outside the beaker, add agar when heated to boil, stir constantly, wait until the agar is completely dissolved, make up the water loss. Adjust the pH to 7.2 ~ 7.4, sterilize after dispensing, and set aside.
Formula 2 Flour agar medium
Flour 60 g Agar 20 g Water 1000 ml Flour the water into a paste, add water to 500 ml, and cook on a simmer for 30 minutes. Another 500 ml of water, add agar, heat and boil until dissolved, mix the two liquids, add water, adjust the pH to 7.4, dispense, sterilize, spare.
3. Fungal medium
Formula One Sabouraud's Medium
Peptone 10 g agar 20 g maltose 40 g water 1000 ml
First, peptone and agar are added with water, heated, and continuously stirred. After the agar is dissolved, 40 g of maltose (or glucose) is added, stirred, dissolved, and then dispensed, sterilized, and set aside.
This culture is commonly used to culture many types of fungi.
Formula 2 Potato Sugar Agar Medium
Wash and peel the potatoes, take 200 grams and cut into small pieces, add 1000 ml of water, boil for half an hour, make up the water. Add 10 g of agar to the filtrate, boil and dissolve, add 20 g of sugar (for the cultivation of mold, add sucrose, add glucose to the yeast), make up the water, dispense, sterilize, and set aside.
The pH of the medium is adjusted to 7.2 to 7.4, and the sugar in the formulation, such as glucose, can also be used to culture actinomycetes and Bacillus.
Formula 3 Soybean bud juice medium
Soybean sprouts 100g agar 15g glucose 20g water 1000ml
Wash the bean sprouts and bring in water for 30 minutes. Filter with gauze, add agar to the filtrate, heat and dissolve, add sugar, stir to dissolve it, make up the water to 1000 ml, dispense, sterilize, and set aside.
The pH of the medium is adjusted to 7.2 to 7.4 and can be used to culture bacteria and actinomycetes.
Formulation four pea agar medium
Pea 80 capsules agar 5 g water 200 ml
Take 80 dried peas and add water, boil for 1 hour, filter with gauze, add agar to the filtrate, boil until dissolved, dispense, sterilize, and set aside.
4, edible fungus culture medium
Formula One Potato-Sucrose-Agar Medium
20% potato boiled juice 1000 ml sucrose 20 g agar 18 g
Wash and peel the potatoes and cut into small pieces. Weigh 200 g of potato pieces, add 1000 ml of water, boil for 20 minutes, and filter. Make up the water in the filter juice to 1000 ml, which is 20% potato juice. Add agar and sucrose to the potato boiled juice, boil it, dissolve it, make up the water, dispense, sterilize, and set aside. The pH is not critical to the use of the medium and may not be measured.
Formula 2 Integrated Potato Medium
20% potato boiled juice 1000 ml
Potassium dihydrogen phosphate 3 g magnesium sulfate 1.5 g glucose 20 g vitamin 10 mg agar 18 g
First prepare 20% potato boiled juice, the same method as above. Add the above various components to the boiled juice, heat and dissolve to make up the water, and adjust the pH to 6. Dispense, sterilize, and spare. The medium is used for cultivating and preserving edible fungus species such as ganoderma lucidum, oyster mushrooms, and shiitake mushrooms.
Formula One Sabouraud's Medium
Peptone 10 g agar 20 g maltose 40 g water 1000 ml
First, peptone and agar are added with water, heated, and continuously stirred. After the agar is dissolved, 40 g of maltose (or glucose) is added, stirred, dissolved, and then dispensed, sterilized, and set aside.
This culture is commonly used to culture many types of fungi.
Formula 2 Potato Sugar Agar Medium
Wash and peel the potatoes, take 200 grams and cut into small pieces, add 1000 ml of water, boil for half an hour, make up the water. Add 10 g of agar to the filtrate, boil and dissolve, add 20 g of sugar (for the cultivation of mold, add sucrose, add glucose to the yeast), make up the water, dispense, sterilize, and set aside.
The pH of the medium is adjusted to 7.2 to 7.4, and the sugar in the formulation, such as glucose, can also be used to culture actinomycetes and Bacillus.
Formula 3 Soybean bud juice medium
Soybean sprouts 100g agar 15g glucose 20g water 1000ml
Wash the bean sprouts and bring in water for 30 minutes. Filter with gauze, add agar to the filtrate, heat and dissolve, add sugar, stir to dissolve it, make up the water to 1000 ml, dispense, sterilize, and set aside.
The pH of the medium is adjusted to 7.2 to 7.4 and can be used to culture bacteria and actinomycetes.
Formulation four pea agar medium
Pea 80 capsules agar 5 g water 200 ml
Take 80 dried peas and add water, boil for 1 hour, filter with gauze, add agar to the filtrate, boil until dissolved, dispense, sterilize, and set aside.
4, edible fungus culture medium
Formula One Potato-Sucrose-Agar Medium
20% potato boiled juice 1000 ml sucrose 20 g agar 18 g
Wash and peel the potatoes and cut into small pieces. Weigh 200 g of potato pieces, add 1000 ml of water, boil for 20 minutes, and filter. Make up the water in the filter juice to 1000 ml, which is 20% potato juice. Add agar and sucrose to the potato boiled juice, boil it, dissolve it, make up the water, dispense, sterilize, and set aside. The pH is not critical to the use of the medium and may not be measured.
Formula 2 Integrated Potato Medium
20% potato boiled juice 1000 ml
Potassium dihydrogen phosphate 3 g magnesium sulfate 1.5 g glucose 20 g vitamin 10 mg agar 18 g
First prepare 20% potato boiled juice, the same method as above. Add the above various components to the boiled juice, heat and dissolve to make up the water, and adjust the pH to 6. Dispense, sterilize, and spare. The medium is used for cultivating and preserving edible fungus species such as ganoderma lucidum, oyster mushrooms, and shiitake mushrooms.
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