Edible fungus mycelium breeds quickly, is easy to mutate, and strains are difficult to preserve. Here are a few methods that work well and are easy to implement. 1. Liquid paraffin preservation method Select liquid paraffin, place it in an incubator for several hours after autoclaving, or in a desiccator for several days. Pipette liquid paraffin with a sterile pipette or syringe and inject a strain of bacteria into the test tube. The injection volume should be about 1 cm above the beveled tip. Plug the tampon and seal it with wax. Store it in an upright position and keep it for 5 years. 2. Bran preservation method Weigh a certain amount of bran, mix it with water or nutrient solution and mix well. Wet bran was packed in test tubes, autoclaved and then inserted into strains and incubated at appropriate temperatures. After the mycelium is well developed, the test tube is dried at room temperature for drying. After drying, it shall be stored in a dry place below 20°C, and it can be stored for 3 to 5 years. 3.Salt preservation method: Put the strains into the potato broth, and add 250 ml of the flask to 60 ml of the culture medium. Shake or shake 5 to 10 times a day. Incubate for 5-7 days. Then move the formed mycelium ball into the equipment. In a test tube containing 5 ml of sterile saline, transfer approximately 4-5 pieces of mycelium into each tube, stuff the tampon, and seal the tube with wax. At low temperatures, it can be stored for 1 year. 4. The wheat preservation method is used to take high-quality wheat, soaked in water at 20° C. for 5 hours after washing, and a little air-dried. The loading capacity is preferably 1/4 to 1/3 of the pipe length. Sterilization, heat and shaking, cooling access to mycelium. Incubate at appropriate temperature until the sparse hyphae appear on most grains. Store in a dry place below 25°C for 1-2 years.
Samsung Battery,Li Ion Battery,Samsung Laptop Battery
Shenzhen Piofond Technology Co., Ltd. , http://www.szliionbattery.com