The ultra-clean workbench is a widely used equipment used by microbiology testing laboratories, and monitoring procedures and instructions should be established.
1. Monitoring procedures: The super-clean workbench should be developed with corresponding monitoring procedures. The main contents are: 1 Regularly monitor the microbiological quality of the inner surface of the ultra-clean workbench and the sterile isolation system and its internal air quality ( Particles and microorganisms require high-efficiency filters to ensure that the rejection of particles greater than or equal to 0.3um is 99.97% or more. The flow rate of laminar air should be sufficient to carry the particles out of the work area. In general, the ideal laminar flow velocity should be controlled between 0.36 and 0.54 m/s on a plane (working area) 15 to 30 cm downstream from the filter; 2 the integrity of the high efficiency filter and the laminar flow rate should be It has been confirmed that the integrity (leak) inspection and filtration efficiency (performance) tests must be performed at the site of use after installation. Such tests must be carried out on a regular basis (usually less than once every six months); 3 once the daily management is found to be unstable, or the environmental monitoring results are abnormal, or the deviation of the microbiological test results is on the rise, an integrity check must be performed immediately. And performance testing. This type of inspection is very important to avoid the potential contamination risks associated with aseptic processing. During aseptic processing, the air quality in the operating area and the microbiological quality of the surface of the console should be monitored at the same time. In the experiment, a positive positive control test should be performed at the same time.
2, User Guide: laminar flow clean bench main content usage guidelines are: walking should be relatively small in the art, and is disposed away from the door position farther laminar flow clean bench is intended to enable the device relative to the ambient air Stable, does not affect the operating area, and must not interfere with the laminar flow in the operating area.
After the equipment is turned on, the operator should first check whether the reading of the differential pressure indicator is normal. Compared with the conventional pressure difference, the pressure difference is small or too large, indicating that the equipment is operating abnormally. It is necessary to investigate immediately and take necessary corrective measures.
If a UV lamp is installed in the operating area, the UV lamp must be turned off to complete all aseptic operations. Prevent UV damage to the operator and damage to microorganisms that may be present in the sample.
The operator can operate in the laminar flow zone after wearing sterile latex gloves and a sterile cuff. After opening the workbench, disinfect the surface with a sterile disinfectant (70% sterile ethanol) and wait for 5 minutes before starting operation. All items placed in the workbench should be thoroughly sterilized and air dried prior to aseptic processing. All items in the work area should be tidy so as not to interfere with the direction of the airflow. The idle items in the workbench should be evacuated immediately and the airflow should not be blocked.
Operators should be familiar with the design, working principles, and airflow patterns of a laminar flow clean bench.
The airflow in the normal laminar flow purification workbench is directly directed to the operator to the surrounding environment. Therefore, such purification workbench should not be used to treat infectious, toxic or sensitive items. They are only suitable for general aseptic handling activities, such as assembly of sterile articles, microbiological examination of conventional articles, preparation of culture media, and the like. Although on the surface, the working flow of the vertical laminar flow purification workbench is not affected by any personnel and the surrounding environment, it is bound to cause potential pollution to the operator and the surrounding environment; the operation should avoid pollution to the surrounding environment and operators. When using a laminar flow table, it is important to ensure that the high efficiency filter is not blocked, thus ensuring that clean air is blown over and covers the entire work area. To prevent disruption of laminar flow, operators should avoid moving their hands and arms in or out of the laminar flow area as much as possible. Even if moving, care should be taken to keep all activities as possible downstream of the exposed area of ​​the sterile item (following the layer). Flow direction). The laboratory is equipped with a purification workbench. It is a biological safety cabinet . It is specially used for the treatment of conventional microbial strains, such as the identification of microorganisms, the preparation and culture of biological indicators, the preparation of standard strains for positive control or medium sensitivity test. , preservation and use.
In the clean area, as far as conditions permit, try to make the laminar flow purification workbench always running. However, once restarted and restarted, the work area should be thoroughly disinfected and less pre-run for 5 minutes before use. In addition, cleaning and disinfection measures should be taken on the work surface before and after each use.
Detailed standard operating procedures should be established for the operation, cleaning and disinfection, calibration and maintenance of various indoor laminar flow tables. Use and maintenance records for each laminar flow table, HTY aseptic isolation system. The contents of the use record include: date and time of use, status before and after the instrument is used, cleaning or disinfection status, and the signature of the user; the maintenance record includes: description of the fault, repair status, and the signature of the maintenance personnel and the person responsible for the equipment. A retrospective review of the equipment's cleaning or disinfection records and environmental monitoring records is performed periodically to assess the maintenance of the work area within the laminar flow table.
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