Wheaton cell culture series CELLine bioreactor working principle and characteristics

Wheaton's CELLine bioreactor uses a cell culture chamber and a medium chamber semi-permeable membrane separation culture technology, which breaks through the constraints of cell culture, air, nutrients and metabolic inhibitors on cell growth. Maximizes the growth environment of cells in an organism. The purpose of high-density cell culture and high-concentration product expression is achieved.

CELLine two-chamber technology schematic

If the CELLine bioreactor is working?

Medium room

The medium chamber can store a large amount of medium. This layer is about 70 times the size of the cell layer and is used to meet the needs of cells for cell culture.

Upper metabolite dialysis membrane

The upper dialysis membrane allows permeation of nutrients having a molecular weight of less than 10 kDa, from the culture chamber to the cell compartment, and the metabolic inhibitors permeate from the cell compartment into the culture chamber and ensure that all proteins of interest remain in the cell compartment.

Cell room

The cell compartment provides an ideal environment for inoculation and collection of high density cultures. This chamber is enriched with high density cells and their intended products.

Lower gas dialysis membrane

Under static culture, the gas delivery rate will limit the density of cell culture. Cells in CELLine culture flasks can obtain oxygen directly from the bottom gas dialysis membrane to balance oxygen and carbon dioxide, increasing gas transfer rates.

Schematic diagram of CELLine bioreactor operation process

Comparison of cell culture volume between Celline bioreactor and traditional T bottle

CELLine bioreactor features

First: high density cell culture

The cell concentration in the CELLine cell chamber is typically 107-108/mL which is two orders of magnitude higher than the normal static cell culture technique.

Second: high product concentration

Thanks to the high cell density, the concentration of CELLine cultured cell products is 1-5mg/mL, which is 50-100 times higher than the traditional culture method.

Third: long-term continuous culture

CELLine's two-chamber culture technology. The user can recycle the product and change the medium. Achieve long-term continuous culture.

Fourth: easy to operate

The CELLine micro cell factory is easy to operate and can be easily cultured using CELLine without adjustment to the original culture system.

Fifth: reduce downstream processing procedures

The two-chamber culture technique allows the cell concentration and the product concentration to be increased by about two orders of magnitude compared with the conventional culture, and the concentration of the enriched culture itself can be directly followed by the subsequent operation without the complicated separation operation of the ordinary culture.

Sixth: cost-effective

The unit yield is high, and the use of CELLine saves a lot of cost compared to traditional T bottles, multi-layer culture plates, etc.

Wheaton PIPET-PALTM electric pipette controller with high frequency motor assisted suction and continuous operation for 6.5 hours at full charge is ideal for cell culture operations.

CO2 incubator

The carbon dioxide incubator is ideal for animal cell culture, germ cell culture, and anaerobic cell culture. Suitable for all microbial culture and hatching experiments, seed cultivation and tissue culture experiments.