Physical and chemical identification
1. Take the endosperm section, add 1 drop of vanadic acid, and show a blue-purple (salmonine reaction); take the slice and add concentrated nitric acid, which is orange-red (streatine reaction).
2. Take 1g of the coarse powder, add ethanol 15ml, cold immersed for 2 hours, the filtrate evaporated, dissolved with 1N hydrochloric acid, ammonium hydroxide alkalinization, chloroform extraction, chloroform solution and then extracted with 1N hydrochloric acid, acid solution Zinc powder and concentrated hydrochloric acid were added and heated in a water bath for 5 minutes. After cooling, 1 drop of nitrous acid solution was added, indicating cherry red (salmonine reaction).
3. TLC: (1) Strychnine. (2) Sapwood alkali. (3) Strychnos substudy (control herbs). (4) Yunnan Maqin (control herbs). (5) Machiko (commodity). (6) Making money.
Sample solution: Take powder 0.5 g, add chloroform-ethanol (10:1) mixture 5 ml and concentrated ammonia solution 0.5 ml, shake for 5 minutes, place for 2 hours, filtrate for test;
Reference liquid: another stramine base, strychnine plus chloroform dissolved, made of each 2 ml each containing 2 mg of mixed solution as a control solution.
Expand: Take 10μl, respectively, on the same silica gel G plate, with toluene - acetone - ethanol - concentrated ammonia solution expansion.
Color: sprayed with potassium bismuth iodide test solution, the control product showed two orange spots, strychnine in the bottom, strychnine at the top, for the test product showed the same stain.
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