One: basic operation steps of the microscope: (for example, fluorescence microscope)
1. Fluorescent light source warms up;
2. In the case where the slide is not placed, adjust the shooting lever to the digital camera for white balance;
3. Adjust the shooting lever to the eyepiece viewing position;
4. Adjust the pupil distance;
5. Adjust the parallax;
6. In the bright field, look for the target under low magnification objective, focus;
7. Change the appropriate objective lens to focus;
8. Adjust the brightness of the light source;
9. Adjust the concentrating mirror;
10. Adjust the filter or aperture as needed
Two: When the clear image is visible in the eyepiece, adjust the microscope to the digital camera as follows:
1. Adjust the shooting lever to transfer the image to the digital camera;
2. Check for focus in bright field. If it is not in focus, turn the fine focus knob to focus. This should be a bright field, keeping the camera at a high frame rate;
3. Adjust the color of the camera, you can do manual white balance as needed, or manually adjust the red, green, and blue gain values;
4. Take a picture. When shooting, in the case of bright field of view, if the normal CMOS camera should have a lower gain value, adjust the saturation as needed. For fluorescence microscopy, the appropriate gain value should be selected according to the recommendation, and the camera frame rate should be lowered to ensure that the noise of the photograph is low;
If you need to take a good picture, the camera should be optimized for microscopy to cool and reduce noise. In addition, the camera preferably has a frame rate that is adjustable to reduce noise.
When imaging under a fluorescence microscope for photo recording, a fluorescence imaging camera that meets the imaging requirements is required.
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