Role study
ã€Objective】 To observe the inhibition of umbilical cord injury induced by oxidized low-density lipoprotein on the proliferation of human umbilical arterial smooth muscle cells.
ã€method】
1 Separate preparation of oxidized low-density lipoprotein.
2. Culture human umbilical vein endothelial cells and arterial smooth muscle cells.
3 Preparation of water extract (Parispolyphyllavar.yunnanensis) (extracted in our laboratory).
4 Using oxidized low-density lipoprotein to stimulate endothelial cell injury to establish a conditioned medium for the proliferation of smooth muscle cells. The experiment was divided into 5 groups: blank control group, conditioned medium group, conditioned medium + 10 μmol/L, and conditioned medium. + 30μmol/L sham group; conditioned medium + 100μmol/L sham group. After different concentrations of sham treatment, cell counting and thymidine incorporation were used to detect drug effects.
ã€result】
1 Compared with the control group, the number of human umbilical arterial smooth muscle cells increased, and the incorporation rate of bismuth-thymidine increased.
2 Compared with the conditioned medium group, both the conditioned medium and the different concentration of cilostazol simultaneously decreased the number of cells and decreased the incorporation rate of thymidine and thymidine, and the DNA synthesis decreased (r = 0.9423, P < 0.05). 3 The inhibitory effect of increasing concentrations of vasopressin on the proliferation of umbilical artery smooth muscle cells increased.
ã€in conclusion】
1 After the injury of endothelial cells, the culture medium can promote the proliferation of smooth muscle cells, and the inhibition of axon-stimulation dose-dependently inhibits the smooth muscle cell count and the amount of thymidine-thymidine incorporation.
2 It can be initially considered that the blood pressure-reducing effect of aphid may be related to its inhibition of human umbilical artery smooth muscle cell proliferation.
[toxic] mice fed decoction 30 ~ 60 g / kg, no deaths within 3 days. The mice were administrated 0.4 g daily for three consecutive days for 3 days, or 0.2% 0.4 ml/v for intravenous injections. No deaths occurred. Therefore, the toxicity is very low.
Plant extracts refer to substances extracted or processed from plants (all or a part of plants) with appropriate solvents or methods, which can be used in pharmaceutical industry, food industry, daily chemical industry and other industries. Nowadays, the application range of plant extracts has been very wide. In addition to traditional Chinese medicine products, with the gradual increase of people's trust and reliance on natural products, a large part of plant extracts have been used in health care products and food ingredients. In addition, plant extracts have been used in cosmetics and feed in recent years.
Andrographis Paniculata Extract,Hydrocotyle Asiatica Extract,Icariin,Reishi Mushroom Extract,Maca Root Extract
Xi'an Gawen Biotechnology Co., Ltd , https://www.amulyn-bio.com